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77 Rapid action of E on GnRH
2022-09-19

Rapid action of E2 on GnRH neurons has been known for some time. E2 hyperpolarizes guinea pig GnRH neurons, alters cAMP production in GT1-cells and stimulates or inhibits [Ca2+]i oscillations within 15 min (Lagrange et al., 1995; Navarro et al., 2003; Temple et al., 2004; Romanò et al., 2008). Impor
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Previous studies showed that pregnancy rate improves
2022-09-19

Previous studies showed that pregnancy rate improves when ewes have a BCS that exceeds a minimum threshold [43]. The pregnancy rate is increased in sheep with BCS ≥2.5 [44] and >2 [45]. In this work, pregnancy rate was higher in the GnRH group with low BCS compared to the hCG and Control groups. Des
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Ion dependence of mGluRs activity has been previously
2022-09-19

Ion dependence of mGluRs activity has been previously reported, notably to Ca2+ and Cl− (Kuang and Hampson, 2006). While mGlu1 and mGlu3 receptors were demonstrated to be sensitive to Ca2+, Cl− modulation was reported for all mGluRs, with a lesser extent for the mGlu2 receptor (DiRaddo et al., 2014;
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To evaluate SLC A abundance after
2022-09-17

To evaluate SLC45A1 abundance after transfection, we performed western blots. Transfected COS-7 WZ3146 were lysed and separated for membrane fraction. Equal amounts of proteins from membrane fractions were resolved by SDS-PAGE, transferred to a nitrocellulose filter, and immunoblotted with an anti-S
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In this study our result suggests
2022-09-17

In this study, our result suggests that exogenous GR agonist DEX and GR inhibitor RU486 can affect dopaminergic neurotransmitters in the brain. Pituitary adenylate cyclase-activating polypeptide (PACAP), an endogenous neuropeptide, can regulate the synthesis and release of catecholamine. McArthur et
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The galactosidase from Aspergillus oryzae is
2022-09-17

The β-galactosidase from Aspergillus oryzae is a monomeric enzyme of 113 kDa [58] that has been widely used in GOS synthesis [49,53,[59], [60], [61]], exhibiting a high activity of lactose transgalactosylation [62]. Different immobilization methods have been used for the synthesis of lactose-derived
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uPAR another newly discovered ligand has implicated FPRL as
2022-09-17

uPAR, another newly discovered ligand, has implicated FPRL1 as a potential link between the fibrinolytic cascade and inflammation. uPA is a serine protease best known for its ability to regulate fibrinolysis and for its importance in tissue remodeling and tumor invasion [49]. However, uPA also induc
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The other type of cell death
2022-09-17

The other type of cell death that occurs in activated T Ouabain is caused by growth factor withdrawal following TCR-mediated activation, whereby most of the activated T cells die by apoptosis following the eradication of bacteria during an acute infection in order to reduce the level of cytokine pr
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br Materials and methods br Results br Discussion In general
2022-09-17

Materials and methods Results Discussion In general, we have demonstrated that the CRISPR/dCas9-based system, fused with 2 × PP7 and PCP-EZH2, could stably and precisely methylate the H3K27 at specific locus of C/ebpα to silence gene expression and inhibit adipogenic differentiation in 3T3
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In summary our results support the model that the
2022-09-17

In summary, our results support the model that the histone demethylase KDM5 is a critical host transcriptional regulator that maintains immune homeostasis and is responsible for preservation of the normal commensal microbial community structure and behavior. Neurodevelopmental disorders such as ID a
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In conclusion we demonstrate for the first time that
2022-09-17

In conclusion, we demonstrate, for the first time, that histamine plays a key role in regulating astrocyte function and gliotransmitter release, and we now must re-consider the roles of monoamine neurotransmitters in brain function in the context of astrocyte signalling. Further in vivo studies will
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br Introduction Adenosine triphosphate ATP is an
2022-09-17

Introduction Adenosine-5′-triphosphate (ATP) is an organic molecule (nucleoside triphosphate) which consists of adenine, ribose, and three phosphoric TAPI-1 residues. ATP serves as a temporary carrier of energy in all living cells, so it is a common substance in any organism. In the cells, new A
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NSC697923 australia Not all metals are susceptible of being
2022-09-16

Not all metals are susceptible of being accumulated by insects. Tissue concentrations of essential metals like Cu and Zn are regulated in many insects and hence cannot be used to biomonitor metal pollution (Hare, 1992). A similar behaviour could explain the observed stability of Zn and Cu relative t
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br Disclosure statement br Introduction Nitro
2022-09-16

Disclosure statement Introduction 1-Nitro-2-phenylethane is the first nitro compound isolated from plants [1]. It is a volatile compound found in the essential oil of various species, and its presence provides a pleasant odor that resembles the cinnamon scent [2]. The biogenesis of 1-nitro-2-p
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br Materials and methods br Results br Discussion To
2022-09-16

Materials and methods Results Discussion To understand the molecular basis for how ghrelin secretion is regulated at the cellular level, we identified the full repertoire of 7TM receptors and G proteins in gastric ghrelin Fucoidan australia and functionally characterized the majority of th
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